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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
21/01/2022 |
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Data da última atualização: |
14/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, A. K. A.; ECHEVERRI, L. M. S.; SOUSA, B. A. de; VICENTE, M. C. de; NASCIMENTO, S. C. do; SILVA, G. F. da; FERREIRA JÚNIOR, A.; LAU, D.; BOGO, A.; SILVA, F. N. da. |
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Afiliação: |
ANA KAROLINY ALVES SANTOS, UDESC/Lages; LINA MARIA SALAZAR ECHEVERRI, UDESC/Lages; BRUNA ANDERSEN DE SOUSA, UDESC/Lages; MONICA CHACON DE VICENTE, UFG; SAMARA CAMPOS DO NASCIMENTO, UDESC/Lages; GUSTAVO FELIPPE DA SILVA, UDESC/Lages; ÁLVARO FERREIRA JÚNIOR, UFG; DOUGLAS LAU, CNPT; AMAURI BOGO, UDESC/Lages; FABIO NASCIMENTO DA SILVA, UDESC/Lages. |
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Título: |
Production and characterization of chicken IgY for detecting wheat stripe mosaic virus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Tropical Plant Pathology, 11 Jan. 2022. |
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DOI: |
https://doi.org/10.1007/s40858-021-00481-x |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant protein was inoculated in laying hens (Gallus gallus domesticus). The anti-WhSMV IgY antibodies were extracted from the chicken yolks. The IgY was the basis for immuno-assays such as indirect Enzyme-linked immunosorbent assay (ELISA) and Western blot, targeting the purifed capsid protein or the whole virus. Also, a PCR test was performed with samples of root and leaves of infected plants, confrming the presence of the virus in both tissues. The PCR results were in agreement with the ELISA tests performed with all samples. IgY produced in chickens specifcally recognized the purifed viral protein and the WhSMV present in the extract from leaves of WhSMV-infected plants. |
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Palavras-Chave: |
Benyviridae; Capsid protein; Criação de frango; Diagnosis; IgY; WhSMV. |
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Thesagro: |
Diagnostico; Mosaico; Trigo; Vírus. |
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Thesaurus Nal: |
Chickens; Triticum aestivum WIS-2 virus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02500naa a2200385 a 4500
001 2139239
005 2022-02-14
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s40858-021-00481-x$2DOI
100 1 $aSANTOS, A. K. A.
245 $aProduction and characterization of chicken IgY for detecting wheat stripe mosaic virus.$h[electronic resource]
260 $c2022
520 $aAbstract: In Brazil, wheat (Triticum aestivum) is cultivated mainly in the southern part. The soilborne wheat mosaic disease (SBWMD), caused by the wheat stripe mosaic virus (WhSMV), is one of the most important viruses in the region. Its diagnosis is based mainly on symptoms since molecular techniques are expensive, especially for RNA genomes viruses. However, serological tests using IgY immunoglobulins are highly efective in identifying viruses. This study aimed to produce and characterize a polyclonal antibody capable of interacting with the WhSMV capsid protein. The gene encoding the virus capsid protein was inserted into the expression vector pET-15b; the transformation and expression occurred in Escherichia coli BL21::DE3 in vitro. The recombinant protein was inoculated in laying hens (Gallus gallus domesticus). The anti-WhSMV IgY antibodies were extracted from the chicken yolks. The IgY was the basis for immuno-assays such as indirect Enzyme-linked immunosorbent assay (ELISA) and Western blot, targeting the purifed capsid protein or the whole virus. Also, a PCR test was performed with samples of root and leaves of infected plants, confrming the presence of the virus in both tissues. The PCR results were in agreement with the ELISA tests performed with all samples. IgY produced in chickens specifcally recognized the purifed viral protein and the WhSMV present in the extract from leaves of WhSMV-infected plants.
650 $aChickens
650 $aTriticum aestivum WIS-2 virus
650 $aDiagnostico
650 $aMosaico
650 $aTrigo
650 $aVírus
653 $aBenyviridae
653 $aCapsid protein
653 $aCriação de frango
653 $aDiagnosis
653 $aIgY
653 $aWhSMV
700 1 $aECHEVERRI, L. M. S.
700 1 $aSOUSA, B. A. de
700 1 $aVICENTE, M. C. de
700 1 $aNASCIMENTO, S. C. do
700 1 $aSILVA, G. F. da
700 1 $aFERREIRA JÚNIOR, A.
700 1 $aLAU, D.
700 1 $aBOGO, A.
700 1 $aSILVA, F. N. da
773 $tTropical Plant Pathology, 11 Jan. 2022.
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Registro original: |
Embrapa Trigo (CNPT) |
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| Registros recuperados : 4 | |
| 1. |  | GROSSI DE SA, M. F.; SOUZA, D. S. L.; ROMANO, E.; SOUSA, B. A. de; GROSSI DE SA, M.; PIRES, N.; ROCHA, T. L.; BARBOSA, A. E. A. D.; SA, C. M.; CARNEIRO, R.; DIAS, S. C.; TEIXEIRA, F. M. High level of resistance to Meloidogyne incognita in transgenic Nicotiana tobacum plants by knockdown of essential oesophagial pionner genes using RNAI. In: INTERNATIONAL CONGRESS ON MOLECULAR PLANT-MICROBE INTERACTIONS, 13., 2007, Sorrento. Book of abstracts... Sorrento: [s. n.], 2007. p.197.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 2. | | SANTOS, A. K. A.; ECHEVERRI, L. M. S.; SOUSA, B. A. de; VICENTE, M. C. de; NASCIMENTO, S. C. do; SILVA, G. F. da; FERREIRA JÚNIOR, A.; LAU, D.; BOGO, A.; SILVA, F. N. da. Production and characterization of chicken IgY for detecting wheat stripe mosaic virus. Tropical Plant Pathology, 11 Jan. 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Trigo. |
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| 3. | | SOUZA, D. dos S. DE L. E; SOUZA JUNIOR, J. D. A. DE; GROSSI DE SÁ, M.; ROCHA, T. L.; FRAGOSO, R. da R.; BARBOSA, A. E. A. DE D.; OLIVEIRA, G. R. DE; NAKASU, E. Y. T.; SOUSA, B. A. DE; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; PINTO, E. R. de C.; ALMEIDA-ENGLER, J. DE; ENGLER, G.; MARTINS-DE-SÁ, C.; SA, M. F. G. de. Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site. Plant Science, v. 180, p. 276-282, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | SOUZA, D. DOS S. DE L. E; SOUZA JUNIOR, J. D. A. DE; GROSSI DE SÁ, M.; ROCHA, T. L.; FRAGOSO, R. da R.; BARBOSA, A. E. A. DE D.; OLIVEIRA, G. R. DE; NAKASU, E. Y. T.; SOUSA, B. A. DE; PIRES, N. F.; DUSI, D. M. de A.; CARNEIRO, R. M. D. G.; PINTO, E. R. de C.; ALMEIDA-ENGLER, J. DE; ENGLER, G.; MARTINS-DE-SÁ, C.; SA, M. F. G. de. Ectopic expression of a Meloidogyne incognita dorsal gland protein in tobacco accelerates the formation of the nematode feeding site. Plant Science, v. 180, p. 276-282, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 4 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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